Background
Europium W1024 isothiocyanate (ITC) is a lanthanide-based fluorophore with a long emission lifetime and large Stokes shift.1,2 It displays excitation/emission maxima of 340/613 nm, respectively.1 Europium W1024 ITC contains a reactive isothiocyanate handle that reacts with primary amino groups at an alkaline pH. It is stable in the presence of high temperature, high EDTA concentration, or low pH but not in the presence of manganese. Europium W1024 ITC has commonly been conjugated to various molecules and used as a donor fluorophore in TR-FRET where its long emission lifetime provides a longer window of measurement for acceptor fluorescence and reduces the interference of autofluorescence.3,4,5 For 's recommended protein labeling protocol please see the product information sheet.
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