Background
2’3’-cGAMP-d8 is intended for use as an internal standard for the quantification of 2’3’-cGAMP by GC- or LC-MS. 2’3’-cGAMP is a second messenger produced from ATP and GTP by cGMP-AMP synthase (cGAS) in the cytoplasm of mammalian cells in response to the presence of dsDNA.1,2,3 It contains a non-canonical 2’5’-phosphodiester bond and binds tightly to the adapter protein stimulator of interferon genes (STING) with a Kd value of 3.79 nM, which is significantly lower than that observed for 2’2’-cGAMP , 3’3’-cGAMP , cyclic di-GMP , and 3’2’-cGAMP (Kds = 0.287, 1.04, 1.21, and 1.61 μM, respectively).3 It is a potent inducer of IFN-β mRNA expression with an EC50 value of 19.4 nM in L929 cells.3
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