A fluorogenic substrate for phosphatases
4-Methylumbelliferyl Phosphate (4-MUP) is a fluorogenic substrate for phosphatases, including acid and alkaline phosphatases.1,2,3 It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively.4
1.Fernley, H.N., and Walker, P.G.Kinetic behaviour of calf-intestinal alkaline phosphatase with 4-methylumbelliferyl phosphateBiochem. J.97(1)95-103(1965) 2.Kohli, A.G., Kieler-Ferguson, H.M., Chan, D., et al.A robust and quantitative method for tracking liposome contents after intravenous administrationJ. Control Release17686-93(2014) 3.Remaley, A.T., Kuhn, D.B., Basford, R.E., et al.Leishmanial phosphatase blocks neutrophil O2 productionJ. Biol. Chem.259(18)11173-11175(1984) 4.Zhi, H., Wang, J., Wang, S., et al.Fluorescent properties of hymecromone and fluorimetric analysis of hymecromone in compound dantong capsuleJ. Spectrosc.147128(2013)
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