(-)-Epicatechin
A polyphenol flavonoid with diverse biological activities
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包装价格促销价数量
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20mg￥662.00530.00- +
- 货号: ajcn5906
- CAS: 490-46-0
- 别名: 表儿茶素
- 分子式: C15H14O6
- 分子量: 290.27
- 纯度: ＞98%
- 溶解度: DMF: 12.5 mg/ml,DMSO: 12.5 mg/ml,DMSO:PBS(pH7.2) (1:1): 0.5 mg/ml
- 储存: Store at -20°C
- 库存: 现货
Background
(-)-Epicatechin is a flavonoid that increases NO levels and an inducer of pancreatic β-cell regeneration [1] [2].
β cells are a subset of cells in the pancreatic islets and play an important role in regulating glucose homeostasis through the production of insulin.
(-)-Epicatechin is an inducer of pancreatic β-cell regeneration. In murine macrophages, (-)-Epicatechin induced nitrite accumulation induced by interferon-γ/lipopolysaccharide with IC50 value of 0.5 mM. Also, (-)-Epicatechin inhibited nitration of free tyrosine induced by 0.1 and 1 mM peroxynitrite with IC50 values of 6.6 and 28.0 μM, respectively. In HL-60 cells, (-)-Epicatechin inhibited nitration of protein-bound tyrosine induced by phorbol 12-myristate 13-acetate with IC50 value of 10-100 μM [2]. In human endothelial cells (HUVEC), (-)-Epicatechin (0.3-10 μM) increased NO levels, suggesting its vasodilatory properties [3].
In alloxan diabetic rats, (-)-Epicatechin (30 mg/kg) lowered blood sugar and restored the amount of beta-cells against alloxan-induced necrosis of the beta-cell population in the pancreatic islets. In rats, (-)-Epicatechin was non-toxic up to 1 g/kg [1]. In rats, oral administration of (-)-Epicatechin metabolized to 3’-O-methyl-(-)-epicatechin and 4’-O-methyl-(-)-epicatechin [4].
参考文献:
[1].? Chakravarthy BK, Gupta S, Gambhir SS, et al. Pancreatic beta-cell regeneration in rats by (-)-epicatechin. Lancet, 1981, 2(8249): 759-760.
[2].? Wippel R, Rehn M, Gorren AC, et al. Interference of the polyphenol epicatechin with the biological chemistry of nitric oxide- and peroxynitrite-mediated reactions. Biochem Pharmacol, 2004, 67(7): 1285-1295.
[3].? Brossette T, Hundsd?rfer C, Kr?ncke KD, et al. Direct evidence that (-)-epicatechin increases nitric oxide levels in human endothelial cells. Eur J Nutr, 2011, 50(7): 595-599.
[4].? Okushio K, Suzuki M, Matsumoto N, et al. Identification of (-)-epicatechin metabolites and their metabolic fate in the rat. Drug Metab Dispos, 1999, 27(2): 309-316.
Protocol
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Animal experiment: |
Mice[3]1-year-old, male C57BL/6N mice (n=25) are randomized into four groups. Mice in the (-)-Epicatechin groups 3 and 4 are given 1.0 mg/kg twice a day (morning and evening) for 15 consecutive days, whereas animals in the control groups 1 and 2 receive the vehicle (water). Both (-)-Epicatechin and vehicle are administered via oral gavage[3]. |
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参考文献: [1]. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9. |
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