Cbz-Gly-Pro-Arg-pNA is a colorimetric substrate for proteases, including thrombin and mast cell tryptase.
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Cbz-Gly-Pro-Arg-pNA is a colorimetric substrate for proteases, including thrombin and mast cell tryptase. Proteases preferentially bind to and cleave the Gly-Pro-Arg (GPR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of protease activity.