LiCl

氯化锂（LiCl）用于沉淀RNA

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Background

IC50: N/A

LiCl is a typical ionic compound, though the small size of the Li+ ion makes its properties not seen for other alkali metal chlorides, such as the extraordinary solubility in polar solvents and hygroscopic properties.

In vitro: Though precipitation with a monovalent cation or alcohol is much more widely used, LiCl has been frequently used to precipitate RNA. LiCl precipitation gives various advantages over other methods: LiCl does not precipitate DNA, protein and carbohydrate efficiently. LiCl can be also used for removing translation inhibitors or cDNA synthesis from RNA preparations. LiCl can be also used as a simple rapid method for recovering RNA from in-vitro transcription reactions [1]. In addition, a method for isolation of large, translationally active RNA samples has been presented. In this study, the procedure included homogenization of samples in 5 m guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 m LiCl. The advantages of this method include simplicity, speed, avoidance of ultracentrifugation, and its applicability to large numbers of samples. The method yielded large mRNA precursors up to 10 kb [2].

In vivo: Currently, there is no animal data reported.

Clinical trial: Up to now, LiCl has not been used clinically.

IC50:不适用

LiCl是一种典型的离子化合物，尽管Li+离子的小尺寸使其性质在其他碱金属氯化物中看不到，例如在极性溶剂中的非凡溶解度和吸湿性。

体外：虽然用单价阳离子或醇沉淀的应用要广泛得多，但LiCl经常用于沉淀RNA。与其他方法相比，LiCl沉淀具有多种优点：LiCl不能有效沉淀DNA、蛋白质和碳水化合物。LiCl也可用于从RNA制剂中去除翻译抑制剂或cDNA合成。LiCl也可以作为一种简单快速的方法从体外转录反应中回收RNA[1]。此外，还提出了一种分离大的翻译活性RNA样品的方法。在本研究中，该程序包括在5 m的单硫氰酸胍中对样品进行均质化，然后用4 m的LiCl从胍中直接沉淀RNA。该方法的优点包括简单、快速、避免超速离心以及适用于大量样品。该方法产生了高达10kb的大的信使核糖核酸前体[2]。

体内：目前尚无动物数据报告。

临床试验：到目前为止，氯化锂尚未在临床上使用。

参考文献:
[1] BARLOW JJ, MATHIAS AP, WILLIAMSON R, GAMMACK DB.? A simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid. Biochem Biophys Res Commun.1963 Sep 10;13:61-6.
[2] Cathala, G.?, Savouret, J., Mendez, B., West, B. L., Karin, M., Martial, J. A., and Baxter, J. D. (1983). "A Method for Isolation of Intact, Translationally Active Ribonucleic Acid".DNA.2(4): 329–335.