Background
PD 146176 is one of the potent and selective inhibitors of reticulocyte 15-LOX-1 [1]. PD 146176 inhibited the activity of h-15-LOX-1 with IC50 value of 16 ± 2.5μM,and Ki value of 3.9 ± 0.6μM [2].
PD 146176 (1 μg ml-1) inhibited the induction of both arginase-1 and mannose receptor mRNA in both rIL-4-treated and RSV-infected WT macrophages, whereas enhancing the induction of COX-2 mRNA [3]. PD 146176 suggested that it causes strong cell cycle arrest in G1 phase. PD 146176 at its IC50, did not show any inhibitory effect on cell directional migration but greatly increased the activity of the caspases in B16F10 cells [4].PD 146176 significantly prevented glutamate-induced cell death in a concentration-dependent manner. PD 146176 fully protected HT-22 cells against glutamate toxicity at a concentration of 0.5 μM and significantly reduced the annexin-V/propidium iodide-positive cells [5].
PD 146176 treated 3xTg mice had significant reductions in Aβ peptide levels, amyloid plaque burden, tau phosphorylation, and insoluble tau deposition in comparison with controls [6,7]. AD model mice in the control group showed a worsening of memory and learning abilities, whereas mice receiving PD 146176 were undistinguishable from wild-type mice [7].
参考文献:
[1]. Orafaie A, Mousavian M, Orafai H, et al. An overview of lipoxygenase inhibitors with approach of in vivo studies[J]. Prostaglandins & Other Lipid Mediators, 2020, 148: 106411.
[2]. Eleftheriadis N, Thee S, Te Biesebeek J, et al. Identification of 6-benzyloxysalicylates as a novel class of inhibitors of 15-lipoxygenase-1[J]. European Journal of Medicinal Chemistry, 2015, 94: 265-275.
[3]. Shirey K A, Lai W, Pletneva L M, et al. Role of the lipoxygenase pathway in RSV-induced alternatively activated macrophages leading to resolution of lung pathology[J]. Mucosal immunology, 2014, 7(3): 549-557.
[4]. Da-Costa-Rocha I, Prieto J M. In vitro effects of selective COX and LOX inhibitors and their combinations with antineoplastic drugs in the mouse melanoma cell line B16F10[J]. International journal of molecular sciences, 2021, 22(12): 6498.
[5]. Tobaben S, Grohm J, Seiler A, et al. Bid-mediated mitochondrial damage is a key mechanism in glutamate-induced oxidative stress and AIF-dependent cell death in immortalized HT-22 hippocampal neurons[J]. Cell Death & Differentiation, 2011, 18(2): 282-292.
[6]. Oddo S, Caccamo A, Shepherd J D, et al. Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Aβ and synaptic dysfunction[J]. Neuron, 2003, 39(3): 409-421.
[7]. Di Meco A, Li J G, Blass B E, et al. 12/15-Lipoxygenase inhibition reverses cognitive impairment, brain amyloidosis, and tau pathology by stimulating autophagy in aged triple transgenic mice[J]. Biological psychiatry, 2017, 81(2): 92-100.
PD 146176 是一种有效的选择性网织红细胞 15-LOX-1 抑制剂[1]。 PD 146176 抑制 h-15-LOX-1 的活性，IC50 值为 16 ± 2.5μM，Ki 值为 3.9 ± 0.6μM [2]。
PD 146176（1 μg ml-1）抑制 rIL-4 处理和 RSV 感染的 WT 巨噬细胞中精氨酸酶-1 和甘露糖受体 mRNA 的诱导，同时增强 COX-2 mRNA 的诱导 [3]. PD 146176 表明它会在 G1 期引起强烈的细胞周期停滞。 IC50 的 PD 146176 对细胞定向迁移没有任何抑制作用，但大大增加了 B16F10 细胞中半胱天冬酶的活性[4]。PD 146176 在一定浓度下显着阻止谷氨酸诱导的细胞死亡-依赖的方式。 PD 146176 在 0.5 μM 浓度下完全保护 HT-22 细胞免受谷氨酸毒性，并显着减少膜联蛋白-V/碘化丙锭阳性细胞[5]。
与对照组相比，PD 146176 处理的 3xTg 小鼠的 Aβ 肽水平、淀粉样斑块负荷、tau 磷酸化和不溶性 tau 沉积显着降低[6,7]。对照组的 AD 模型小鼠表现出记忆和学习能力的恶化，而接受 PD 146176 的小鼠与野生型小鼠没有区别[7]。
Protocol
| Cell experiment [1]: |
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Cell lines
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murine melanoma cell line B16F10
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Preparation Method
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1 × 104 cells per well were seeded in each well of a 96-well tissue culture plate and left overnight in the incubator. The cells were incubated with PD 146176 for 24, 48, or 72 h similar to the MTT assay.
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Reaction Conditions
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0.5, 1, 2 , 10, 20, 40μM for 24, 48, 72 hours
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Applications
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PD 146176 induced cell cycle arrest in G1 phase at 8 h with decrease of cells in S and G2/M phase. The effect on cell morphology is visible observed after 16 h with cells becoming smaller and rounded.
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| Animal experiment [2]: |
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Animal models
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C57BL/6 mice
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Preparation Method
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One group was untreated and ate ground rodent chow for 7 days while the experimental group was fed ground rodent chow with the selective PD 146176 added at a concentration to achieve a dose of about 400 mg/kg/day
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Dosage form
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Fed with diet , 400 mg/kg/day, 7 days
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Applications
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The mice that were fed PD 146176 lost significantly more weight at 3-5 days after starting dextran sodium sulfate, compared to the corresponding day for the control mice.
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参考文献:
[1]: Da-Costa-Rocha I, Prieto J M. In vitro effects of selective COX and LOX inhibitors and their combinations with antineoplastic drugs in the mouse melanoma cell line B16F10[J]. International journal of molecular sciences, 2021, 22(12): 6498.
[2]: Joshi Y B, Giannopoulos P F, Praticò D. The 12/15-lipoxygenase as an emerging therapeutic target for Alzheimer's disease[J]. Trends in pharmacological sciences, 2015, 36(3): 181-186.
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