NHS-Biotin

Biotinylation reagent

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库存: 现货

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100mg

￥350.00

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￥2475.00

1980.00

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货号: ajci11250
CAS: 35013-72-0
别名: (+)生物素-N-琥珀酰亚胺基酯,Biotin N-hydroxysuccinimide ester, Biotin-OSU, Biotin NHS, Biotin SE
分子式: C14H19N3SO5
分子量: 341.38
纯度: >98%
溶解度: ≥ 17.069mg/mL in DMSO
储存: Desiccate at -20°,protect from light,unstable in solution, ready to use.
库存: 现货

Background

NHS-Biotin (C₁₄H₁₈O₅N₃S) is N-hydroxysuccinimido biotin. It is the shortest of three similar EZ-Link NHS-Biotin Reagents that enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing biomolecules in solution. NHS-Biotin offers researchers the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Because it is uncharged and contain simple alkyl-chain spacer arms, NHS-Biotin compound is membrane-permeable and useful for intracellular labeling.

NHS-Biotin is the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.

NHS-Biotin（C14H18O5N3S）是N-羟基琥珀酰亚胺生物素。它是三种相似的EZ-Link NHS-Biotin试剂之一，可在溶液中对抗体、蛋白质和任何其他含有一级胺基的生物分子进行简单有效的生物素化。NHS-Biotin为研究人员提供了优化标记和检测实验的可能性，其中生物素结合的空间阻碍是一个重要因素。由于它是不带电的，并含有简单的烷基空间臂，因此NHS-Biotin化合物是可渗透膜的，并且对于细胞内标记非常有用。

NHS-Biotin是最流行的生物素化试剂类型之一。NHS活化的生物素与碱性缓冲液中的一级氨基（-NH2）高效反应，形成稳定的酰胺键。蛋白质（例如抗体）通常具有几个可用于标记的一级胺基，包括赖氨酸（K）残基的侧链和每个多肽的N-末端。

Reference:
[1]. Fouassier, L., Yun, C. C., Fitz, J. G. and Doctor, R. B. (2000). Evidence for Ezrin-Radixin-Moesin-binding Phosphoprotein 50 (EBP50) Self-association through PDZ-PDZ Interactions. J. Biol. Chem. 275, 25039-25045.
[2]. Nunomura, W., Takakuwa, Y., Parra, M., Conboy, J.G., & Mohandas, N. (2000) Regulation of protein 4.1R, p55 and glycophorin C ternary complex in human erythrocyte membrane. J. Biol. Chem. 275(32): 24540-24546.
[3].Chiu, N.H. & T.K. Christopoulos. 1999. Two-site expression immunoassay using a firefly luciferase-coding DNA label. Clin. Chem. 45: 1954–1959.

Protocol

Biotinylation method [1]:
Sample	whole blood
Preparation method	Soluble in DMSO or DMF.
Reaction Conditions	30 minutes
Applications	Firstly, NHS-biotin was dissolved in DMSO at a concentration of 100 mg/mL, after that using 90% DMSO solution diluted the biotin to a concentration of 20 mg/mL. The biotin solution was sterilized by ?ltering the solution through 0.2-μm pore nylon syringe ?lters and the solution was diluted with 6 volumes 0.9% saline solution. Blood was allowed to warm to room temperature prior to labeling with NHS-biotin. To achieve a biotin concentration of 0.04 pg of biotin/RBC（Red Blood Cells）, Biotin-DMSO-saline solution was injected into each blood bag and agitated for 30 minutes.
参考文献: [1]. J.E. Tomlinson, E. Taberner, R.C. Boston, S.D. Owens, and R.D. Nolen-Walston. Survival Time of Cross-Match Incompatible Red Blood Cells in Adult Horses. J Vet Intern Med 2015;29:1683–1688.