7-Ethoxyresorufin

A fluorogenic substrate for, and inhibitor of, CYP1A1

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库存: 现货

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数量
5mg

￥762.00

610.00

- +
10mg

￥1300.00

1040.00

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25mg

￥2725.00

2180.00

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50mg

￥4850.00

3880.00

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100mg

￥8100.00

6480.00

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货号: ajci11810
CAS: 5725-91-7
别名: 乙氧基试卤灵,Resorufin ethyl ether
分子式: C14H11NO3
分子量: 241.24
纯度: >98%
溶解度: Chloroform: Soluble,DMF: Soluble,DMSO: Soluble
储存: Store at -20°C
库存: 现货

Background

Cytochrome P450, family 1, subfamily A, polypeptide 1 is a protein that is encoded by human CYP1A1 gene. The protein is a member of the cytochrome P450 superfamily of enzymes. CYP1A1 is involved in phase I xenobiotic and drug metabolism. CYP1A1 is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. 7-Ethoxyresorufin is a specific subtrate of CYP1A.

In vitro: 7-Ethoxyresorufin (2-10 microM), a substrate for cytochrome P450, which binds to the heme moiety of the enzyme, reduced responses to acetylcholine concentration-dependently but not the other agonists indicating an effect on nitric oxide synthesis although neither affected the conversion of L-arginine to L-citrulline [1].

In vivo: Activities of CYP1A (7-ethoxyresorufin) in the liver was determined for comparative purposes. In mice, LPS depressed CYP2A5 at low doses but not at doses that increased pro-inflammatory cytokines and NO serum levels, and depressed CYP1A activity. Blockade of proinflammatory cytokines extended down-regulation of CYP2A5 while not affecting LPS-induced depression of CYP1A [2].

Clinical trial: Cytochrome P-450 enzyme activities of 7-ethoxyresorufin O-deethylase (ERDE) was measured in human liver needle biopsy samples from smokers and non-smokers. ERDE activity was significantly elevated in the livers of cigarette smokers. No correlation was observed between plasma cotinine concentration and ERDE activity [3].

参考文献:
[1] Oyekan AO, McGiff JC, Rosencrantz-Weiss P, Quilley J.? Relaxant responses of rabbit aorta: influence of cytochrome P450 inhibitors. J Pharmacol Exp Ther. 1994 Jan;268(1):262-9.
[2] De-Oliveira AC, Po?a KS, Totino PR, Paumgartten FJ.? Modulation of cytochrome P450 2A5 activity by lipopolysaccharide: low-dose effects and non-monotonic dose-response relationship. PLoS One. 2015 Jan 30;10(1):e0117842.
[3] Pelkonen O, Pasanen M, Kuha H, Gachalyi B, Kairaluoma M, Sotaniemi EA, Park SS, Friedman FK, Gelboin HV.? The effect of cigarette smoking on 7-ethoxyresorufin O-deethylase and other monooxygenase activities in human liver: analyses with monoclonal antibodies. Br J Clin Pharmacol. 1986 Aug;22(2):125-34.