10Panx

A peptide inhibitor of PANX1

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库存: 现货

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价格

促销价

数量
1mg

￥550.00

440.00

- +
5mg

￥1487.00

1190.00

- +
10mg

￥2712.00

2170.00

- +
25mg

￥6025.00

4820.00

- +

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金额: ￥0.00

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Background

10Panx is a mimetic inhibitory peptide of panx1, which is a mammalian protein ectopically expressed to function as a hemi-channel, that readily and reversibly inhibits the panx1 currents. 10Panx has attracted increasing attention as it allows scientists readily and reliably to examine inhibition of panx1 in cells where efficient and consistent delivery of siRNA is highly difficult. According to results of multiple previous studies, 10Panx selectively inhibits P2X₇R-induced dye uptake without altering other aspects of R2X₇R activation and ATP-evoked membrane currents, constitutive dye-uptake and hemichannel-like currents that are induced by the overexpression of panx1, and ATP-mediated IL-1β release.

Reference

[1].Pablo Pelegrin and Annmarie Surprenant. Pannexin-1 mediates large pore formation and interleukin-1β release by the ATP-gated P2X₇ receptor. The EMBO Journal (2006) 25, 5071-5082

Protocol

Cell experiment: [1]
Cell lines	Human red blood cells (RBCs)
Preparation method	The solubility of this peptide in sterile water is >10 mM. Stock solution should be splited and stored at -80°C for several months.
Reaction Conditions	200 μM,
Applications	10Panx reduced ATP release in response to lowered pO2 by 90.9±15.5%. It did not reduce ILO-induced ATP release.
Animal experiment:
Animal models
Dosage form
Applications
Other notes	Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
参考文献: [1] Sridharan M, Adderley S, Bowles E, et al. Pannexin 1 (PANX1) is a Conduit for ATP Released from Human Red Blood Cells (RBCs) Exposed to Low Oxygen Tension. The FASEB Journal, 2010.