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A fluorescent probe
Coumarin hydrazine is an aromatic hydrazine-containing fluorophore that reacts with aldehydes or ketones for fluorescent labeling. At neutral pH, coumarin hydrazine reacts faster with aldehydes than coumarin hydrazide to form hydrazones. Coumarin hydrazine acts as a fluorogenic sensor in live cells to detect carbonylated biomolecules that occur following oxidative stress. It displays an excitation maximum of 365 nm, produces a red shift in emission from approximately 430-550 nm upon hydrozone formation, and displays a large Stokes shift of approximately 195 nm.
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