CAY10732

CAY10732 functions as a fluorescent agent designed to identify the presence of carbon monoxide.

此产品仅用于科学研究，我们不为任何个人用途提供产品和服务

库存: 现货

可选规格

包装

价格

促销价

数量
1mg

￥500.00

400.00

- +
5mg

￥2275.00

1820.00

- +
10mg

￥3975.00

3180.00

- +
50mg

￥17275.00

13820.00

- +

已选 0 种 0 瓶

金额: ￥0.00

首页收藏

货号: ajci70654
CAS: 855751-82-5
别名:
分子式: C26H20O5
分子量: 412.4
纯度: >98%
溶解度: DMF: 12 mg/ml,DMSO: 10 mg/ml,Ethanol: 1 mg/ml
储存: Store at -20°C
库存: 现货

Background

CAY10732 functions as a fluorescent agent designed to identify the presence of carbon monoxide.1 When exposed to palladium (Pd) and carbon monoxide, CAY10732 initiates the Pd0-mediated Tsuji-Trost reaction, resulting in the liberation of 2,7-dichlorofluorescein. The fluorescence emitted by this compound serves as an indicator of carbon monoxide production, both within controlled laboratory settings and within living cells. The excitation/emission peaks of 2,7-dichlorofluorescein are measured at 493/527 nm, respectively^[1].

CAY10732是一种检测一氧化碳的荧光探针。在钯(Pd)和一氧化碳存在下，CAY10732通过pd0介导的Tsuji-Trost反应释放2,7-二氯荧光素，其荧光可用于体外和活细胞中检测一氧化碳的产生。2,7-二氯荧光素的激发/发射最大值分别为493/527 nm^[1]。

参考文献:

[1]. Feng, S, Liu, D, et al. Allyl fluorescein ethers as promising fluorescent probes for carbon monoxide imaging in living cells. Anal. Chem. 89(6), 3754-3760 (2017).

Protocol

Protocol for Imaging of CO in Living Cells^[1]：

Stock Solution Preparation：Stock solutions of CAY10732 (2 mM), PdCl₂ (2 mM), and CORM-3 (10 mM) were separately prepared in HPLC grade DMSO and used freshly. CAY10732 (1 μM) and PdCl₂ (1 μM) in PBS buffer (10 mM, pH 7.4, with 0.5% DMSO, v/v) was prepared in use.

1.HeLa cells were seeded in a 24-well culture plate for one night before cell imaging experiments.

2.In a typical experiment of cell imaging, as controls, living cells were incubated with CAY10732 (1 μM) and a mixture of CAY10732 and PdCl₂ (1 μM each) at 37℃ for 30 min, respectively, and they were imaged after washing with PBS for three times.

3.For imaging of exogenous CO, HeLa cells were pretreated with CORM-3 (1, 5, and 10 μM, respectively) for 30 min at 37℃ and then were incubated with a mixture of CAY10732 and PdCl₂ (1 μM each) for 30 min at 37℃. After washing the cells with PBS buffer, the cells were imaged.

4.For imaging of heme stimulation produced CO, HeLa cells were pretreated with heme (100 μM) for 30 min at 37℃ and then were incubated with a mixture of CAY10732 and PdCl₂ (1 μM each) for 30 min at 37℃. After washing the cells with PBS buffer, the cells were imaged.

This protocol only provides a guideline, and should be modified according to your specific needs.

参考文献:

[1]. Feng, S, Liu, D, et al. Allyl fluorescein ethers as promising fluorescent probes for carbon monoxide imaging in living cells. Anal. Chem. 89(6), 3754-3760 (2017).