Luminol (Diogenes reagent)

A chemiluminescent probe

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Background

Luminol is a cyclic diacyl hydrazide that exhibits chemiluminescence upon oxidation. For example, HRP, combined with hydrogen peroxide as an activator, causes luminescent peroxidation of luminol.¹ This reaction can be enhanced by certain phenol derivatives, such as p-substituted phenols.² Luminol can also be oxidized, and chemiluminesce, by compounds containing iron, copper, gold, and cyanide.^3,4 The excitation/emission maxima for luminol are 355/411 nm.

1.Cormier, M.J., and Prichard, P.M.An investigation of the mechanism of the luminescent peroxidation of luminol by stopped flow techniquesJ. Biol. Chem.243(18)4706-4714(1968) 2.Thorpe, G.H.G., Kricka, L.J., Moseley, S.B., et al.Phenols as enhancers of the chemiluminescent horseradish peroxidase-luminol-hydrogen peroxide reaction: Application in luminescence-monitored enzyme immunoassaysClin. Chem.31(8)1335-1341(1985) 3.Fan, A., Cao, Z., Li, H., et al.Chemiluminescence platforms in immunoassay and DNA analysesAnal. Sci.25(5)587-597(2009) 4.Vladimirov, Y.A., and Proskurnina, E.V.Free radicals and cell chemiluminescenceBiochemistry (Mosc.)74(13)1545-1566(2009)