Phenol Red sodium salt (Phenolsulfonephthalein sodium salt)

Phenol Red sodium salt (Phenolsulfonephthalein sodium salt) is used ubiquitously as a pH indicator in tissue culture media ranging from 6.8 (yellow) to 8.2 (red). Phenol red binds to the estrogen receptor of MCF-7 human breast cancer cells.

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5g

￥600.00

480.00

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货号: ajce44712
CAS: 34487-61-1
别名: 酚红钠,Phenolsulfonephthalein sodium salt
分子式: C19H13NaO5S
分子量: 376.36
纯度: >98%
溶解度: Water : ≥ 100 mg/mL (265.70 mM)
储存: Store at 4°C, protect from light
库存: 现货

Background

[1] Y Berthois, et al. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500.

Protocol

Cell experiment:

Phenol red is dissolved in 0.1% ethanol. To determine the effect of phenol red on cell proliferation, MCF-7 cells grown for 1 week before experiments in phenol red-free MEM supplemented are harvested and seeded into T-25 flasks (ca. 1.5 x 105 cells per flask). The following day, cells from three flasks are harvested and counted with a Coulter Counter. Then the medium is changed to phenol red- and insulin-free MEM, which contains various concentrations of dextran-coated charcoal-treated calf serum, phenol red (0-300 μM), tamoxifen, hydroxytamoxifen, estradiol, or ethanol vehicle (0.1%), and cell number is monitored as a function of time[1]. Phenol Red sodium salt is dissolved in distilled water as a 10 g/L stock solution[2].

参考文献:

[1]. Berthois Y, et al. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2496-500.
[2]. Pick E, et al. A simple colorimetric method for the measurement of hydrogen peroxide produced by cells in culture. J Immunol Methods. 1980;38(1-2):161-70.