Forchlorfenuron

A plant growth regulator

此产品仅用于科学研究，我们不为任何个人用途提供产品和服务

库存: 现货

可选规格

包装

价格

促销价

数量
50mg

￥262.00

210.00

- +
100mg

￥375.00

300.00

- +
500mg

￥550.00

440.00

- +
1g

￥650.00

520.00

- +

已选 0 种 0 瓶

金额: ￥0.00

首页收藏

Background

Forchlorfenuron is a plant growth regulator.^1,2 It stimulates fruit growth, as well as increases fruit size and ripening rate, in kiwifruit (A. chinensis) when applied topically at a concentration of 40 mg/L.¹ Forchlorfenuron accelerates fruit growth and increases rind thickness in watermelons (C. lanatus).² Formulations containing forchlorfenuron have been used to increase fruit growth in agricultural applications.

1.Iwahori, S., Tominaga, S., and Yamasaki, T.Stimulation of fruit growth of kiwifruit, Actinidia chinensis Planch., by N-(2-chloro-4-pyridyl)-N'-phenylurea, a diphenylurea-derivative cytokininSci. Hort.35(1-2)109-115(1988) 2.Kano, Y.Effects of CPPU treatment on fruit and rind development of watermelons (Citrullus lanatus Matsum. et Nakai)J. Hortic. Sci. Biotechnol.75(6)651-654(2000)

Protocol

Cell experiment:

CHO cells are cultured in high-glucose DMEM medium. Forchlorfenuron and its kiwifruit metabolites are dissolved in DMSO and diluted in culture media. Cells are seeded in a 96-well flat microtiter plate for 24 h and are then incubated for an additional 48 h with various concentrations of the compounds. Next, cells are fixed with 10% trichloroacetic acid and incubated for 60 min at 4 °C. The supernatant is discarded, then the plates are washed. Using 0.4% SRB solution dissolved in 1% acetic acid, the cell layer is strained, then the plates are incubated for 20 min at room temperature. The stained cells is solubilized in 10 mM un-buffered Tris base and optical density (OD) is measured at 560 nm[1].

参考文献:

[1]. Zhang Z, et al. Identification, synthesis, and safety assessment of forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) and its metabolites in kiwifruits. J Agric Food Chem. 2015 Mar 25;63(11):3059-66.
[2]. Chen W, et al. Dissipation and residue of forchlorfenuron in citrus fruits. Bull Environ Contam Toxicol. 2013 Jun;90(6):756-60.