MCLA hydrochloride

A superoxide probe

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库存: 现货

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数量
1mg

￥1287.00

1030.00

- +
5mg

￥2637.00

2110.00

- +

已选 0 种 0 瓶

金额: ￥0.00

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货号: ajce45318
CAS: 128322-44-1
别名: 2-甲基-6-(4-甲氧苯基)-3,7-二氢咪唑并[1,2-A]吡嗪-3-酮盐酸盐[化学发光试剂]
分子式: C14H14ClN3O2
分子量: 291.73
纯度: >98%
溶解度: DMF: 10 mg/ml,DMSO: 5 mg/ml,Ethanol: 2 mg/ml,PBS (pH 7.2): 5 mg/ml
储存: Store at -20°C
库存: 现货

Background

MCLA is a Cypridina luciferin analog that is used to detect superoxide generated by activated leukocytes and macrophages.¹ It emits chemiluminescence near 654 nm by reaction with superoxide or singlet oxygen.¹

1.Takahashi, A., Nakano, M., Mashiko, S., et al.The first observation of O2- generation in in situ lungs of rats treated with drugs to induce experimental acute respiratory distress syndromeFEBS Lett.261(2)369-372(1990)

Protocol

Kinase experiment:

The standard reaction mixture contains 1×l0-7 M MCLA hydrochloride (MCLA), 5×10-5 M hypoxanthine, XOD (6.5 U), SOD (0.2 to 20 ng/mL) or none, and 50 mM Tris-HCl buffer containing 0.1 mM EDTA at pH 7.8, in a total volume of 3.0 mL. Chemiluminescence measurement is initiated by the addition of MCLA hydrochloride to the standard incubation mixture excluding XOD, continued for 4 min without additive and for an additional 4 min after the addition of XOD. Chemiluminescence is measured using a luminescence reader at 25°C[1].

参考文献:

[1]. Kimura H, et al. Highly sensitive and reliable chemiluminescence method for the assay of superoxide dismutase inhuman erythrocytes. FEBS Lett. 1988 Nov 7;239(2):347-50.