Sodium ionophore III (ETH2120)|81686-22-8|安捷凯

Background

Sodium ionophore III (ETH2120) is a Na+ ionophore.

Cell suspensions of Acetobacterium woodii prepared from cultures grown on fructose plus caffeate catalyzed caffeate reduction with electrons derived from molecular hydrogen. The sodium ionophore Sodium ionophore III (ETH2120), stimulated hydrogen-dependent caffeate reduction by 280%, indicating that caffeate reduction is coupled to the buildup of a membrane potential generated by primary Na(+) extrusion^[1]. Rnf is a membrane protein complex, The addition of the protonophore TCS strongly inhibited lactate-sulfate dependent growth whereas the sodium ionophore Sodium ionophore III (ETH2120) had no effect, indicating a role for the proton gradient during growth. ^[2]. In the considered nitrobenzene medium, the investigated Sodium ionophore III ligand is a very effective receptor for the Eu3+ and Am3+ cations^[3].

The addition of the Na+-selective ionophore Sodium ionophore III (ETH2120) or the protonophore CCCP or the H+/cation-antiporter monensin revealed that an H+ gradient is used as primary energy conservation mechanism, which strengthens the exceptional position of C. aceticum as acetogenic bacterium showing an H+-dependent energy conservation mechanism as well as Na+-dependent growth^[4].

参考文献:
[1]: Imkamp F, Müller V. Chemiosmotic energy conservation with Na(+) as the coupling ion during hydrogen-dependent caffeate reduction by Acetobacterium woodii. J Bacteriol. 2002 Apr;184(7):1947-51. doi: 10.1128/JB.184.7.1947-1951.2002. PMID: 11889102; PMCID: PMC134933.
[2]: Wang L, Bradstock P, et,al. The role of Rnf in ion gradient formation in Desulfovibrio alaskensis. PeerJ. 2016 Apr 14;4:e1919. doi: 10.7717/peerj.1919. PMID: 27114876; PMCID: PMC4841214.
[3]: Makrlík, E., Kví?alová, M. et,al.Sodium Ionophore III as Very Effective Receptor for Trivalent Europium and Americium. J Solution Chem 45, 463-474 (2016). https://doi.org/10.1007/s10953-016-0447-0
[4]: Mayer A, Weuster-Botz D. Reaction engineering analysis of the autotrophic energy metabolism of Clostridium aceticum. FEMS Microbiol Lett. 2017 Dec 1;364(22). doi: 10.1093/femsle/fnx219. PMID: 29069379.

钠离子载体 III (ETH2120) 是一种 Na+ 离子载体。

从在果糖和咖啡酸盐上生长的培养物制备的 Acetobacterium woodii 的细胞悬浮液通过来自分子氢的电子催化咖啡酸盐还原。钠离子载体钠离子载体 III (ETH2120) 刺激氢依赖性咖啡酸减少 280%，表明咖啡酸减少与初级 Na(+) 挤出产生的膜电位的建立有关^[1]。 Rnf 是一种膜蛋白复合物，质子载体 TCS 的添加强烈抑制乳酸-硫酸盐依赖性生长，而钠离子载体钠离子载体 III (ETH2120) 没有影响，表明质子梯度在生长过程中的作用。 ^[2]。在所考虑的硝基苯介质中，所研究的钠离子载体 III 配体是 Eu3+ 和 Am3+ 阳离子的非常有效的受体^[3]。

Na+- 选择性离子载体钠离子载体 III (ETH2120) 或质子载体 CCCP 或 H+/阳离子逆向转运蛋白莫能菌素的添加表明，H+ 梯度被用作主要的能量守恒机制，这加强了醋酸梭菌的特殊地位具有 H+- 依赖性能量守恒机制和 Na+- 依赖性生长的产乙酸菌^[4].

Protocol

Cell experiment [1]:

Cell lines

Acetobacter xylostella cells

Preparation Method

After the cell suspensions were gassed with H2 for 30 min at 30 ?ムin a shaking water bath at 180 rpm, caffeate was added as indicated in the figure legends from an 0.1 M stock solution. The ionophores N,N, Sodium ionophore III (ETH2120), tetrachlorosalicylanilide (TCS), 2-(3,5-di-tert-butyl-4-hydroxy-benzylidene)-malononitrile (SF6487), and the ATPase inhibitor N,N -dicyclohexylcarbodiimide (DCCD) were added as ethanolic solutions as indicated in the figure legends

Dosage form

20 μM ETH2120 for 24h

Applications

Preincubation of the cells with the Na+ ionophore sodium ionophore III not only stimulated caffeate reduction(by Sodium ionophore III (ETH2120)), but completely abolished ATP synthesis. Addition of sodium ionophore III to cells in the steady state of caffeate reduction immediately dissipated the intracellular ATP level^[1].

参考文献:

[1]: Imkamp F, M¨1ller V. Chemiosmotic energy conservation with Na(+) as the coupling ion during hydrogen-dependent caffeate reduction by Acetobacterium woodii. J Bacteriol. 2002 Apr;184(7):1947-51. doi: 10.1128/JB.184.7.1947-1951.2002. PMID: 11889102; PMCID: PMC134933.