Sucrose (D-(+)-Saccharose)

Sucrose (D-(+)-Saccharose), a common sugar produced naturally in plants, is a disaccharide, a molecule composed of two monosaccharides:glucose and fructose.

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Background

Sucrose (D-(+)-Saccharose), a common sugar produced naturally in plants, is a disaccharide, a molecule composed of two monosaccharides:glucose and fructose.

[1] C Foyer, et al. Arch Biochem Biophys. 1983 Jan;220(1):232-8.

Protocol

Animal experiment:

A total of 66 male 8-wk-old Sprague-Dawley obesity-prone (OP) and obesity-resistant (OR) rats (n?=?38 per each phenotype), weighing 275 g and 210 g respectively, are used in this study. After recovery and attaining pre-surgical weights, rats undergo three training trials of 60-min sham feeding sessions with 0.03 M Sucrose solution. During testing, rats are briefly deprived of water (0900 to 1600 h) but not food, and are tested for 1 h (1400 to1500 h) sham intake of 0.03 M Sucrose solution until a stable baseline is achieved. Afterwards, rats are sham fed one of three Sucrose solutions (0.03, 0.3, and 1.0 M) in random order, every other day, with each concentration tested at least twice. Sucrose intake is individually recorded every 5 min for 60 min[1].

参考文献:

[1]. Duca FA, et al. Effect of diet on preference and intake of sucrose in obese prone and resistant rats. PLoS One. 2014 Oct 20;9(10):e111232.