Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin)

A randomly methylated form of β-cyclodextrin

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货号: ajce49636
CAS: 128446-36-6
别名: 甲基-β-环糊精; Methyl-beta-cyclodextrin
分子式:
分子量: 1310(Average)
纯度: >98%
溶解度: DMSO : ≥ 100 mg/mL;Water : ≥ 50 mg/mL
储存: Store at 4°C
库存: 现货

Background

Methyl-β-cyclodextrin is a randomly methylated form of the cyclic oligosaccharide β-cyclodextrin .¹ Methyl-β-cyclodextrin contains seven D-(+)-glucopyranose units that contain randomized hydrogen or methyl groups. Methyl-β-cyclodextrin has been used to improve the aqueous solubility of various compounds and to extract cholesterol from lipid membranes.^1,2 Methyl-β-cyclodextrin (5 mM) reduces α-synuclein levels in the membrane and detergent-insoluble fractions from B103 neuroblastoma cells transfected with human α-synuclein.³ It also reduces α-synuclein levels in mouse brain in a transgenic model of α-synucleinopathy.

甲基-β-环糊精是环糊精β的随机甲基化形式。1甲基-β-环糊精含有七个D-(+)-葡萄糖吡喃糖单元，其中包含随机的氢或甲基基团。甲基-β-环糊精已被用于改善各种化合物的水溶性，并从脂质膜中提取胆固醇。1,2甲基-β-环糊精（5 mM）可以降低人类α-突触核蛋白在B103神经母细胞转染体的膜和洗涤剂不溶性部分中的水平。3它还可以降低转基因α-突触核蛋白病模型小鼠大脑中α-突触核蛋白的水平。

1.Katageri, A.R., and Sheikh, M.A.Cyclodextrin a gift to pharmaceutical world reviewInt. Res. J. Pharm.3(1)52-56(2012) 2.Besenicar, M.P., Bavdek, A., Kladnik, A., et al.Kinetics of cholesterol extraction from lipid membranes by methyl-β-cyclodextrin--a surface plasmon resonance approachBiochim. Biophys. Acta1778(1)175-184(2008) 3.Bar-On, P., Rockenstein, E., Adame, A., et al.Effects of the cholesterol-lowering compound methyl-β-cyclodextrin in models of α-synucleinopathyJ. Neurochem.98(4)1032-1045(2006)

Protocol

Cell experiment:

PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1].

Animal experiment:

Mice: Female NRJ mice are intraperitoneally inoculated with BCBL-1 cells suspended in PBS. The mice are then treated with intraperitoneal injections of PBS or methyl-β-cyclodextrin (500 mg/kg per day). Tumor burdens are evaluated by measuring body weights and ascites[1].

参考文献:

[1]. Mundhara N, et al. Methyl-β-cyclodextrin, an actin depolymerizer augments the antiproliferative potential of microtubule-targeting agents. Sci Rep. 2019 May 21;9(1):7638.
[2]. Chen X, et al. Cholesterol depletion from the plasma membrane triggers ligand-independent activation of the epidermal growth factor receptor. J Biol Chem. 2002 Dec 20;277(51):49631-7.
[3]. Gotoh K, et al. The antitumor effects of methyl-β-cyclodextrin against primary effusion lymphoma via the depletion of cholesterol from lipid rafts. Biochem Biophys Res Commun. 2014 Dec 12;455(3-4):285-9.
[4]. Tiwari G, et al. Cyclodextrins in delivery systems: Applications. J Pharm Bioallied Sci. 2010 Apr;2(2):72-9.