Fluorescein Diacetate (3,6-Diacetoxyfluoran)

A cell-permeable fluorogenic cell viability probe

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5g

￥400.00

320.00

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货号: ajce51108
CAS: 596-09-8
别名: 荧光素二乙酸酯; 3,6-Diacetoxyfluoran; Di-O-acetylfluorescein
分子式: C24H16O7
分子量: 416.38
纯度: >98%
溶解度: DMSO : 26.67 mg/mL (64.05 mM)
储存: Store at -20°C,protect from light
库存: 现货

Background

Fluorescein diacetate is a cell-permeable fluorogenic probe to quantify cell viability.¹ It is cleaved by intracellular esterases to produce fluorescein which displays excitation/emission maxima of 490/520 nm, respectively.²

1.Brunius, G.Technical aspects of the use of 3',6'-diacetyl fluorescein for vital fluorescent staining of bacteriaCurr. Microbiol.4321-323(1980) 2.Dive, C., Cox, H., Watson, J.V., et al.Polar fluorescein derivatives as improved substrate probes for flow cytoenzymological assay of cellular esterasesMol.Cell Probe2(2)131-145(1988)

Protocol

Cell experiment:

MCF7 cells (2-3×105) are seeded in a 35-mm glass bottom dish before the experiment. Prior to imaging, cells are washed with 1 mL PBS, then incubated in 1 mL Hanks’ Balanced Salt solution (HBSS(+) without phenol red) containing 1 μM Fluorescein diacetate (0.1% DMSO as a cosolvent) for 5 min at 37°C. Cells are washed twice with 1 mL PBS and 1 mL HBSS is added before imaging. Fluorescence images of fluorescein and DsRed-Express2 were acquired in the FITC channel (excitation at 473 nm) and the DsRed channel (excitation 559 nm). The 16-bit images obtained are analysed[2].

参考文献:

[1]. Boyd V, et al. Limitations in the Use of Fluorescein Diacetate/Propidium Iodide (FDA/PI) and Cell PermeableNucleic Acid Stains for Viability Measurements of Isolated Islets of Langerhans. Curr Trends Biotechnol Pharm. 2008 Mar;2(2):66-84.
[2]. Fujikawa Y, et al. Fluorescein diacetate (FDA) and its analogue as substrates for Pi-class glutathione S-transferase (GSTP1) and their biological application. Talanta. 2018 Mar 1;179:845-852.