7-Aminoactinomycin D (7-AAD)

A fluorescent DNA dye

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1mg

￥1862.00

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货号: ajce51396
CAS: 7240-37-1
别名: 7-氨基放线菌素D; 7-AAD
分子式: C62H87N13O16
分子量: 1270.43
纯度: >98%
溶解度: DMF: 10 mg/ml,DMF:PBS(pH 7.2)(1:1): 0.5 mg/ml,DMSO: 5 mg/ml
储存: -20°C, protect from light,unstable in solution, ready to use.
库存: 现货

Background

7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells.^1,2 It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm.³ As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC.^1,3 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.^1,2,4

1.Schmid, I., Uittenbogaart, C.H., Keld, B., et al.A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometryJ. Immunol. Methods170(2)145-157(1994) 2.Coder, D.M.Assessment of cell viabilityCurr. Protoc. Cytom.Suppl. 15(1997) 3.Stokke, T., Holte, H., and Steen, H.B.In vitro and in vivo activation of B-lymphocytes: A flow cytometric study of chromatin structure employing 7-aminoactinomycin DCancer Res.48(23)6708-6714(1988) 4.Lecoeur, H., Février, M., Garcia, S., et al.A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicityJ. Immunol. Methods253(1-2)177-187(2001)