Janelia Fluor 669, SE (JF669, SE) 是一种红色荧光染料,可在温和条件下 (DIEA, DMF) 直接与可用的含硫醇 HaloTag 配体反应,一步即可得到 JF669?HaloTag 配体(Ex \u003d 669 纳米;Em \u003d 682 纳米)。
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Red fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag? and SNAP-tag?. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Cell permeable. Excitation maximum = 669 nm; emission maximum = 682 nm; Quantum yield = 0.37; Extinction coefficient = 116,000 M-1cm-1; Correction factor = 0.0430. Retains 97% fluorescence after 30 bleaching cycles. We also offer Janelia Fluor? conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.
Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757
2',7'-Dichlorofluorescein diacetate
¥470.00 ¥587.00
Pentafluorobenzenesulfonyl fluorescein
¥540.00 ¥675.00
Cy3 NHS ester (non-sulfonated)
¥1420.00 ¥1775.00
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