Background
Adenosine-5'-O-diphospho-(1''-(4-nitrophenoxy)-ribose) is a nitrophenyl ribose-modified nucleotide and colorimetric substrate for poly(ADP-ribose) polymerase 1 (PARP1).1 PARP1 binds and drolyzes adenosine-5'-O-diphospho-(1''-(4-nitrophenoxy)-ribose) to release p-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of PARP1 activity.
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